primary human dermal lymphatic endothelial cells lecs  (PromoCell)

Journal: Advanced Healthcare Materials

Article Title: A Human‐Based Skin‐Lymphoreticular Model‐on‐Chip to Emulate Inflammatory Skin Conditions

doi: 10.1002/adhm.202503170

Figure Lengend Snippet: Integration of lymphatic endothelial cells (LEC) increases the biomimicry of a self‐assembled lymphoreticular (LR) model. A) Exemplary histological staining of a human lymph node. Scale bar = 1 mm. Copyright permission has been obtained from Xinxiang Happy Science Co., Ltd. B) Schematic illustration of the layering approach. C) Brightfield image of a self‐assembled LR model. D) Representative H&E staining and E) IF images showing the FRC‐derived ECM marker ER‐TR7 (red), DAPI (blue) and the localization of GFP‐tagged LECs of LR models generated using different layering approaches: FLF (FRCs + GPF‐LECs + FRC), FLFN (FRC + GPF‐LECs + (FRC + naïve CD4 + T cells)), FLFA (FRC + GPF‐LECs + (FRC + activated CD4 + T cells)), FLN (FRC + GPF‐LECs + naïve CD4 + T cells), and FLA (FRC + GPF‐LECs + activated CD4 + T cells). Circles and arrows indicate the compartmentalization within LR models. Semi‐quantification of F) ER‐TR7 expression and G) GFP‐LEC distribution in the LR models generated using the different layering approaches. n = 3. One‐way ANOVA followed with Tukey post‐hoc tests were performed. Mean ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ****p ≤ 0.0001. H) IF staining against collagen type 1 and fibronectin as key ECM components of lymphoid tissue in FLFA models. Cell nuclei are counterstained with DAPI (blue). Scale bars = 100 µm.

Article Snippet: Green fluorescent protein‐expressing dermal primary human lymphatic endothelial cells (GFP – LECs) (cAP‐0003GFP, male donor) and the Quick Coating Solution (cAP‐01) were purchased from Angio‐Proteomie.

Techniques: Staining, Derivative Assay, Marker, Generated, Expressing